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目的检测脑忆源片中淫羊藿苷在大鼠血浆中的血药浓度,计算其药动学参数。方法采用HPLC法测定不同时间点淫羊藿苷的血药浓度,药-时曲线数据经DAS药动学计算程序处理。色谱柱:Phenomenex lunaC18(150 mm×4.60 mm,5μm);流动相∶乙腈-水(30∶70);流速:1 mL.min-1;检测波长:270 nm。结果淫羊藿苷在0.25~13μg/mL之间呈良好的线性关系(r=0.9996);大鼠灌胃脑忆源片后,淫羊藿苷的药-时曲线符合二室一级吸收模型,主要动力学参数:t1/2α(1.554)h;t1/2β(1.692)h;ka(0.463)h-1;Tmax(3)h;Cmax(4.423875)mg.L-1;AUC0~t(25.877)mg.L-1.h-1;AUC0~∞(25.889)mg.L-1.h-1。结论本法准确、灵敏、简便,适用于淫羊藿苷的血药浓度测定,为含淫羊藿制剂的药动学研究及质量控制提供了参考依据。
Objective To detect the plasma concentration of icariin in the brain of rats with brain-derived tablets, and calculate its pharmacokinetic parameters. Methods HPLC method was used to determine the plasma concentration of icariin at different time points. The data of drug-time curve was processed by DAS pharmacokinetic program. Column: Phenomenex lunaC18 (150 mm × 4.60 mm, 5 μm); mobile phase: acetonitrile-water (30:70); flow rate: 1 mL.min-1; detection wavelength: 270 nm. Results Icariin showed a good linearity between 0.25 ~ 13μg / mL (r = 0.9996). The pharmacokinetics-time curve of icariin was in accordance with the two-compartment first-order absorption model , The main kinetic parameters were t1 / 2α (1.554) h, t1 / 2β (1.692) h, ka (0.463) h-1, Tmax (3) h, Cmax (4.423875) mg.L-1, AUC0 ~ t 25.877) mg.L-1.h-1; AUC0 ~ ∞ (25.889) mg.L-1.h-1. Conclusion The method is accurate, sensitive and simple and suitable for the determination of Icariin. It provides a reference for pharmacokinetics and quality control of Epimedium preparations.