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目的 观察转基因细胞Tca81 1 3/TNF α的致突变作用 ,将转基因肿瘤细胞作为“瘤苗”的临床应用的安全性提供依据。方法 利用基因转染技术 ,用逆转录病毒LXSN·TNF α载体系统 ,将肿瘤坏死因子基因 (TNF α基因 )转导入人舌癌Tca81 1 3细胞 ,通过遗传毒理学Ames实验对转基因的Tca81 1 3/TNF α细胞的细胞DNA及其培养上清液进行体外的致突变试验研究。结果 (1 )DNA组菌落数最大值 (x±s) :1 50 5± 1 1 2 / 1 60 2± 7 6 (TA97) ;34 0± 2 2 / 34 4± 3 2 (TA98) ;1 39 8± 4 6/ 1 30 8± 7 2 (TA1 0 0 ) ;2 64 0± 1 4 6/ 2 54 4± 8 2 (TA1 0 2 )。 (2 )培养上清液组菌落数最大值 (x±s) :1 4 0 5± 1 1 2 / 1 55 2± 7 6 (TA97) ;34 0± 1 0 / 36 4± 7 2 (TA98) ;1 4 5 6± 5 6/ 1 64 4± 6 2(TA1 0 0 ) ;2 54 0± 6 0 / 2 84 4± 8 2 (TA1 0 2 )。结论 TNF α基因转导Tca81 1 3细胞不会引起基因突变 ,转基因细胞作为“瘤苗”的应用初步评价为安全可靠的
Objective To observe the mutagenic effect of Tca81 1 3 / TNFα in transgenic cells and to provide evidence for the safety of transgenic tumor cells as a “tumor vaccine” for clinical application. Methods Tumor necrosis factor gene (TNFα) gene was transduced into human tongue cancer Tca81 1 3 cells by gene transfection technique using retrovirus LXSN · TNFα vector system. The gene Tca81 1 3 / TNF a cell DNA and its culture supernatant in vitro mutagenicity test study. Results (1) The maximum number of colonies in DNA group (x ± s): 1 50 5 ± 1 1 2/1 60 2 ± 7 6 (TA97); 34 0 ± 2 2/34 4 ± 3 2 (TA98); 1 39 8 ± 4 6/1 30 8 ± 7 2 (TA1 0 0); 2 64 0 ± 1 46/2 54 4 ± 8 2 (TA1 0 2). (2) The maximum number of colonies in the culture supernatant group was (x ± s): 1 4 0 5 ± 1 1 2/1 55 2 ± 7 6 (TA97); 34 0 ± 1 0/36 4 ± 7 2 ); 1 546 ± 5 6/1 64 4 ± 6 2 (TA1 0 0); and 2 54 0 ± 6 0/2 84 4 ± 8 2 (TA1 0 2). CONCLUSION: TNFα gene transduction of Tca81 1 3 cells will not cause gene mutation. The application of transgenic cells as “tumor vaccine” was initially evaluated as safe and reliable