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从■(Prunus salicina Lindl.var.cordataJ.Y.Zhang et al.)成熟叶片均一化全长cDNA文库中新分离得到了1个PPO基因,命名为PsPPO2,基因登录号为JF681036.1。经NCBI-BLAST分析,其核苷酸序列与蔷薇科果树中的河北鸭梨和富士苹果果实的PPO基因具有很高的同源性,分别为81%和80%;与作者先前分离克隆的PsPPO1和PsPPO3基因同源性分别为55%和56%,说明此克隆是PPO基因家族的新成员。通过RT-PCR分析,PsPPO1、PsPPO2和PsPPO这3个基因在■不同生长发育时期和果实受损伤后的表达模式。在叶片发育过程中,PsPPO2表达量都很高;PsPPO1在嫩芽和幼叶中表达;PsPPO3只在嫩芽中表达;在果实发育的不同时期,PsPPO2和PsPPO3在早期表达,随着果实成熟表达量下降,PsPPO1在褐变果中表达量较高。受机械损伤后,PsPPO1受诱导,而PsPPO2和PsPPO3不受诱导。
One PPO gene was newly isolated from the mature full-length cDNA library of Prunus salicina Lindl.var.cordataJ.Y.Zhang et al. And named PsPPO2. The accession number was JF681036.1. The NCBI-BLAST analysis showed that the nucleotide sequence was 81% and 80% homologous to the PPO gene of Hebei pear and Fuji apple in the Rosaceae fruit tree, respectively. Compared with the previously isolated and cloned PsPPO1 And PsPPO3 were 55% and 56%, respectively, indicating that this clone is a new member of PPO gene family. By RT-PCR analysis, PsPPO1, PsPPO2 and PsPPO these three genes in different growth stages and fruit damage expression patterns. PsPPO1 was expressed in shoots and young leaves; PsPPO3 was only expressed in shoots; PsPPO2 and PsPPO3 were expressed in the early stages of fruit development and expressed as mature fruits The amount of PsPPO1 was higher in browning fruit. After mechanical injury, PsPPO1 was induced, while PsPPO2 and PsPPO3 were not induced.