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应用Disector方法观察缺氧缺血性脑损伤新生大鼠脑选择性易损区海马CA1区锥体细胞层神经元形态计量学改变。结果显示,与正常对照组相比,缺氧缺血后三周新生大鼠海马CA1区锥体细胞层神经元数密度减少38%,体密度减少47%,表面积密度减少33%,比表面增加25%,圆球度减少16%,统计学均有显著性差异。而细胞核平均体积及平均表面积的改变无统计学意义。Disector为三维测试系统,可直接在三维空间计数粒子,不受粒子大小、形状、取向的影响。适当选择切片厚度及相邻两切片间的距离,可无偏地估计神经元数目及其大小。
Disector method was used to observe the morphological changes of pyramidal cell neurons in the hippocampal CA1 pyramidal neurons of selective hypoxic-ischemic brain damage area in neonatal rats with hypoxic-ischemic brain damage. The results showed that compared with the normal control group, the density of neurons in hippocampal CA1 pyramidal cell layer decreased 38%, the body density decreased 47%, the surface area density decreased 33% and the specific surface increased 25%, the degree of sphericity decreased by 16%, statistically significant differences. The average volume of the nucleus and the average surface area of the change was not statistically significant. Disector is a three-dimensional test system that counts particles directly in three dimensions regardless of particle size, shape, or orientation. Appropriate choice of slice thickness and the distance between two adjacent slices can be unbiased to estimate the number of neurons and their size.