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目的:观察海带多糖对高脂饮食性大鼠主动脉内皮型一氧化氮合酶(eNOS)、诱导型一氧化氮合酶(iNOS)与基质金属蛋白酶(MMP)-2、MMP-9表达的影响,探讨海带多糖抗高脂饮食诱导血管病变的作用与机制。方法:采用高脂饲料联合维生素D3诱导大鼠高脂血症。造模成功后,将大鼠随机分为正常组、模型组、辛伐他汀2 mg/kg、海带多糖低剂量(250 mg/kg)、中剂量(500 mg/kg)、高剂量(1000 mg/kg)治疗组。给药8周后,颈动脉取血测定血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)的含量;测定血清与主动脉中NO和一氧化氮合酶(NOS)的含量。取大鼠主动脉,蛋白免疫印迹法检测eNOS、iNOS、MMP-2和MMP-9的蛋白表达,RT-PCR法分析eNOS、iNOS与MMP-2、MMP-9的基因表达。结果:与模型组相比,海带多糖在1000 mg/kg剂量下能显著降低血清中TC、TG、LDL-C的含量,同时也可升高HDL-C的含量;在500 mg/kg剂量下能够降低TG的含量,提高HDL-C的含量。海带多糖治疗能够显著恢复高脂饮食性引起的血清和主动脉NO和NOS含量的降低。此外,海带多糖在1000 mg/kg剂量下能显著抑制主动脉iNOS与MMP-2、MMP-9的表达,上调eNOS的蛋白表达;在500 mg/kg剂量下能明显降低血管iNOS并升高eNOS的表达。结论:海带多糖除改善高脂饮食性血清脂质谱,提高血清和大动脉NO及NOS含量外,对高脂饮食性大鼠主动脉eNOS、iNOS与MMP-2、MMP-9表达有再平衡作用。
Objective: To observe the effects of laminarin on the expression of eNOS, iNOS, MMP-2 and MMP-9 in the aorta of high-fat diet rats Influence, explore the role of kelp polysaccharide against high-fat diet induced vascular lesions and mechanism. Methods: Hyperlipidemia was induced by high fat diet combined with vitamin D3. After successful modeling, the rats were randomly divided into normal group, model group, simvastatin 2 mg / kg, low dose of kelp polysaccharide 250 mg / kg, medium dose 500 mg / kg and high dose 1000 mg / kg) treatment group. After 8 weeks of administration, the content of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) Serum and aorta were measured NO and nitric oxide synthase (NOS) content. The aortas of rats were taken out and the protein expressions of eNOS, iNOS, MMP-2 and MMP-9 were detected by Western blotting. The gene expressions of eNOS, iNOS, MMP-2 and MMP-9 were analyzed by RT- Results: Compared with the model group, the kelp polysaccharide at the dosage of 1000 mg / kg could significantly reduce the levels of TC, TG and LDL-C in serum and increase the content of HDL-C at 500 mg / kg Can reduce the content of TG, increase HDL-C content. Laminaria polysaccharide treatment can significantly reduce high-fat diet-induced serum and aortic NO and NOS decreased. In addition, kelp polysaccharide at a dose of 1000 mg / kg significantly inhibited aorta iNOS and MMP-2, MMP-9 expression, upregulation of eNOS protein expression; at 500 mg / kg dose can significantly reduce blood vessel iNOS and increased eNOS expression. Conclusion: The kelp polysaccharide can restrain the expression of eNOS, iNOS, MMP-2 and MMP-9 in the aorta of rats with high-fat diet in addition to improving the lipid profile of high-fat diet and the content of NO and NOS in serum and aorta.