论文部分内容阅读
目的以汉坦病毒重组核衣壳蛋白(NP)为抗原,对陕西、河北两省部分地区HFRS患者的血清进行ELISA分型。方法用5种汉坦病毒NP为抗原,建立一种间接ELISA法,并检测上述地区患者血清中特异性IgG。结果陕西、河北两省部分地区汉坦病毒的感染以血清型HTN为主,其次为SEO型,并检测出2例疑似DOB型感染的血清。结论用汉坦病毒NP作抗原进行ELISA血清分型是可行的,并首次发现我国存在DOB血清型的免疫学证据。
Objective To detect the serum of HFRS patients in some areas of Shaanxi Province and Hebei Province by using recombinant retrovirus nucleocapsid protein (NP) as antigen. Methods Five kinds of Hantavirus NPs were used as the antigen to establish an indirect ELISA method and to detect the specific IgG in serum of patients in the above areas. Results The prevalence of Hantaviruses in some areas of Shaanxi and Hebei provinces was serotype HTN, followed by SEO type, and two cases of suspected DOB infection were detected. Conclusion It is feasible to use the Hantaan virus NP as an antigen for ELISA serotyping, and for the first time found that there is immunological evidence of DOB serotype in our country.