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通过免疫组化方法观测胃溃疡黏膜组织中表皮生长因子及其受体的表达,分析健脾化浊方保护消化性溃疡胃黏膜的作用机制。方法:参照Okabe的方法并加以改进造模,成功后将其随机分为空白对照组、健脾化浊方组和雷尼替丁组,每组各8只,另设正常对照组8只,连续灌胃14天后取大鼠胃黏膜溃疡灶或溃疡瘢痕及其周围组织,用免疫组化法观测组织中EGF和EGF表达。结果:健脾化浊方促进EGF及其受体EGFR的表达,提高再生黏膜的功能成熟度,从而提高QOHU,可能是其促进溃疡愈合的机制之一。
The expression of epidermal growth factor and its receptor in gastric ulcer mucosa was observed by immunohistochemical method and the mechanism of action of Jianpi Huazhuo Fang in protecting gastric mucosa of peptic ulcer was analyzed. Methods: According to Okabe’s method and improve the model, after successful, it was randomly divided into blank control group, Jianpi Huazhuo Decoction group and ranitidine group, with 8 rats in each group and 8 normal control animals. After 14 days of continuous gavage, gastric mucosal ulcer lesion or ulcer scar and its surrounding tissues were obtained, and the expression of EGF and EGF was observed by immunohistochemistry. Results: Jianpi Huazhuo Fang can promote the expression of EGF and its receptor EGFR, improve the functional maturation of regenerative mucosa, and improve QOHU, which may be one of the mechanisms of its promoting ulcer healing.