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目的 业已证明 ,醛固酮可以促进纤维化的形成 ,血管、脑及肝脏等肾上腺外组织能够合成醛固酮。本实验意在研究醛固酮合成酶基因—CYP11B2在正常与肝纤维化大鼠肝组织中的表达 ,评价安体舒通的治疗作用。方法 取雄性Wistar大鼠 160只 ,体重 2 50克左右 ,随机分为 4组 ,每组 4 0只。模型组 :CCl4 油 0 2 5ml 10 0mg皮下注射 ,每周三次 ;安体舒通组 :CCl4 油注射的同时予以安体舒通 2 0mg·kg 1·d 1灌胃 ,1次 日 ;马洛替酯组 :CCl4 油注射的同时予以马洛替酯 50mg·kg 1·d 1灌胃 ,1次 日 ;对照组 :橄榄油皮下注射。于第 2、4、6、8和 10周宰杀动物 ,光镜及电镜下动态观察组织学改变 ,图像分析仪测量胶原面积。RT PCR和原位杂交检测CYP11B2在正常及纤维化大鼠肝脏的表达。结果 原位杂交及RT PCR显示CYP11B2表达定位于肝星形细胞胞浆 ,在纤维化形成时表达增强。第四、六周 ,安体舒通组肝纤维化分级、胶原面积低于模型组 (P <0 0 5) ,安体舒通组与马洛替酯组无明显差异 (P >0 0 5)。第八、十周 ,安体舒通组肝纤维化分级 ,胶原面积高于马洛替酯组 (P <0 0 5)。安体舒通组与模型组相比差异无显著意义 (P >0 0 5)。结论 CYP11B2在纤维化肝脏的星形细胞中表达增强。安体舒通对早期肝纤维化具有一定的?
Purpose It has been shown that aldosterone can promote the formation of fibrosis, blood vessels, brain and liver and other adrenal tissue synthesis of aldosterone. The purpose of this experiment is to investigate the expression of aldosterone synthase gene-CYP11B2 in liver tissues of normal and hepatic fibrosis rats and to evaluate the therapeutic effect of spironolactone. Methods 160 male Wistar rats weighing 250 g were randomly divided into 4 groups with 40 rats in each group. Model group: CCl4 oil 0 2 5ml 10 0mg subcutaneously three times a week; spironolactone group: CCl4 oil injection while spironolactone 20mg · kg 1 · d 1, 1 day; Marlow For the ester group: CCl4 oil injection at the same time to marxit 50 mg · kg 1 · d 1 gavage, 1 day; control group: olive oil subcutaneous injection. Animals were sacrificed at 2, 4, 6, 8, and 10 weeks, and histological changes were observed under light and electron microscopy. Image analysis was used to measure collagen area. The expression of CYP11B2 in normal and fibrotic rat liver was detected by RT PCR and in situ hybridization. Results In situ hybridization and RT-PCR showed that the expression of CYP11B2 localized in the cytoplasm of hepatic stellate cells and its expression was enhanced during the formation of fibrosis. In the fourth and sixth weeks, the liver fibrosis grade and collagen area of spironolactone group were lower than that of the model group (P <0.05), there was no significant difference between spironolactone group and malodotian group (P> 0.05) ). In the eighth and tenth weeks, the spironolactone group was graded with liver fibrosis and the collagen area was higher than that of the malotitis group (P <0.05). There was no significant difference between the spironolactone group and the model group (P> 0.05). Conclusion CYP11B2 is expressed in astrocytes of fibrotic liver. Spironolactone on the early liver fibrosis have a certain?