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目的探讨白细胞介素-1(IL-1)基因多态性与尘肺易感性的关系。方法以80例Ⅰ期矽肺和45例Ⅰ期煤工尘肺为病例,以与病例年龄相差小于5岁、同性别、同民族、同一工作地点、开始接尘时间和累积接尘工龄相差不超过2年的非尘肺接尘工人为对照,进行1∶1配对。采集静脉血,用酚-氯仿法抽提DNA,采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)和PCR方法检测IL-1α(-889)、IL-1β(-511)、1L-1 Ra(+2018)和IL-1Ra数目可变的串联重复序列4个基因位点的基因型,并进行单因素、多因素分析。结果两组1L-1α(-889)1/2+2/2基因型的携带率的差异有统计学意义(P＜0．01),病例组与对照组IL-1α(-889)等位基因2的频率分别为57．6%和40．8%,差异有统计学意义(P＜0．01),多因素分析表明,IL-1α(-889)突变基因型及等位基因2为尘肺的危险因素。病例组与对照组之间IL-1β(-511)1/2+2/2基因型、IL-1 Ra(+2018)1/2+2/2基因型和IL-1Ra VNTR1/2+2/ 2基因型携带率的差异均无统计学意义(P＞0．05),病例组与对照组IL-1β(-511)、IL-1 Ra(+2018)和IL-1Ra VNTR等位基因2的频率差异无统计学意义(P＞0．05)。矽肺组和煤工尘肺组间4个等位基因及基因型分布频率差异均无统计学意义(P＞0．05)。结论IL-1α(-889)基因多态性与尘肺患病有关,携带IL-1α(-889)等位基因2的接尘者患尘肺的危险性增加,未发现IL-1β(-511)、IL-1Ra (+2018)及IL-1 Ra VNTR基因多态性与尘肺发病有关联。
Objective To investigate the relationship between interleukin-1 (IL-1) gene polymorphism and susceptibility to pneumoconiosis. Methods Eighty cases of Stage I silicosis and 45 cases of Stage I coal worker’s pneumoconiosis were enrolled in this study. The difference between their age and sex was less than 5 years, same sex, same ethnicity, same place of work, Year non-pneumoconiosis dust workers as a control, 1: 1 matching. The venous blood was collected and DNA was extracted by phenol-chloroform method. The levels of IL-1α (-889), IL-1β (-511) and IL-1β were detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) 1L-1 Ra (+2018) and IL-1Ra variable number of tandem repeats of the four gene loci genotypes, and univariate, multivariate analysis. Results There was a significant difference in the carrying rates of 1L-1α (-889) 1/2 genotype between the two groups (P <0.01). IL-1α (-889) The frequency of gene 2 was 57.6% and 40.8%, respectively, with significant difference (P <0.01). Multivariate analysis showed that the genotype of IL-1α (-889) and allele 2 were Pneumoconiosis risk factors. IL-1β (-511) 1/2 genotype, IL-1 Ra (+2018) 1/2 genotype and IL-1Ra VNTR1 / 2 + 2 genotypes between the case group and the control group (P <0.05). The IL-1β (-511), IL-1 Ra (+2018) and IL-1Ra VNTR alleles in case group and control group were significantly higher than those in control group 2 frequency difference was not statistically significant (P> 0.05). There were no significant differences in the frequencies of the four alleles and genotypes between silicosis group and coal worker pneumoconiosis group (P> 0.05). Conclusion The polymorphism of IL-1α (-889) is associated with pneumoconiosis. The risk of pneumoconiosis was increased in those exposed to IL-1α (-889) allele 2, and IL-1β (-511) , IL-1Ra (+2018) and IL-1 Ra VNTR gene polymorphisms associated with pneumoconiosis.