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【目的】确定圆唇散白蚁Reticulitermes labralis补充生殖蚁不同发育阶段表皮长链碳氢化合物(cuticular longchained hydrocarbons,CHCs)的变化。【方法】人工隔离巢群饲养圆唇散白蚁R.labralis,观测其翅芽型补充生殖蚁的分化时间和补充生殖蚁数量趋于稳定的时间,并采用气质联用仪(GC-MS)对补充生殖蚁不同发育阶段的表皮长链碳氢化合物变化趋势进行分析。【结果】随着补充生殖蚁的出现,每一巢群会逐渐形成稳定数量的具有生殖能力的生殖蚁。若蚁在分化为补充生殖蚁的第8天时,43.3%的组补充生殖蚁数量趋于稳定,第16天时83.3%的组趋于稳定,第25天时93.3%的组趋于稳定,36 d后100%的组趋于稳定。通过补充生殖蚁不同发育阶段中CHCs各峰面积与若蚁阶段相应峰峰面积的比值随发育阶段的变化趋势分析发现,在卵中没有检测到的保留时间为38.52min的化合物在补充生殖蚁产7~8粒卵时峰面积比值是若蚁阶段的14.14倍;在卵中检测到的保留时间为35.50和39.21 min的峰面积比值在补充生殖蚁开始产卵后显著升高。【结论】CHCs不能用来区分有无生殖能力的补充生殖蚁个体,但可以很好地用来区分补充生殖蚁个体与尚未转化为生殖蚁的若蚁个体;保留时间为38.52 min的化合物是补充生殖蚁的特有物质;保留时间为35.50和39.21 min的化合物可作为补充生殖蚁已经产卵的标志。
【Objective】 To determine the changes of cuticular longchained hydrocarbons (CHCs) in Reticulitermes labralis at different developmental stages. 【Method】 Artificial isolation of R. rufipalus was used to keep R. ruralis, the differentiation time of reproductive ants and the number of reproductive ants were observed. The GC-MS method Analysis of the changes of epidermal long-chain hydrocarbons in different stages of reproductive development. 【Result】 With the advent of complementary reproductive ants, each nest will gradually form a stable number of reproductive ants. When the ants were differentiated into reproductive ants on the 8th day, 43.3% of the group replenished the number of reproductive insects stabilized on the 16th day, 83.3% of the group tends to stabilize on the 25th day 93.3% of the group tends to stabilize, 36 days after 100% of the group tends to be stable. By complementing the ratio of peak areas of CHCs in different developmental stages of reproductive ants to the corresponding peaks and peaks of ants in the developmental stages, we found that there was no detectable retention time of 38.52 minutes in the eggs The peak area ratio of 7 to 8 eggs was 14.14 times of that of the ant stage. The retention times detected in the eggs were 35.50 and 39.21 minutes, respectively, and the peak area ratio increased significantly after the primiparous reproductive period began to lay eggs. 【Conclusion】 CHCs can not be used to distinguish reproductive individuals with or without reproductive abilities, but they can be used to distinguish reproductive ants from individuals that have not yet been transformed into reproductive ants. Compounds that retain a retention time of 38.52 min are supplemented The reproductive ants unique substances; retention time of 35.50 and 39.21 min of the compound can be used as reproductive antlers have spawned a sign.