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用中性红摄入和３Ｈ－ＴｄＲ掺入抑制法两种体系建立了定量测定肿瘤杀伤和肿瘤生长因子活性的方法。其关键是对脾ＬＡＫ细胞与Ｙａｏ－１细胞共培养后的上清样品进行倍比稀释，并将使Ａｎｉｐ肿瘤细胞杀伤率或３Ｈ－ＴｄＲ掺入抑制率增加或减少５０％时的上清量作为一个活性单位。结果表明在本实验中杀伤率和３Ｈ－ＴｄＲ掺入抑制率之间并无一致性。
Methods for the quantitative determination of tumor killer and tumor growth factor activity were established using both neutral red intake and 3H-TdR incorporation inhibition systems. The key is to double-dilute the supernatant sample of the spleen LAK cells co-cultured with Yao-1 cells, and to increase or decrease the killing rate of Anip tumor cells or the inhibition rate of 3H-TdR incorporation by 50%. As a living unit. The results showed no consistency between the killing rate and the 3H-TdR incorporation inhibition rate in this experiment.