目的 :采用高效液相色谱法测定人血浆中酮洛芬浓度。方法 :用石油醚 -乙醚 ( 1∶1)提取血浆样品中酮洛芬及萘普生 (内标 ) ,在SpherisorbC18柱上 ,以甲醇 - 5 0mmol·L-1磷酸二氢钠 ( 63∶3 7)为流动相进行分离 ,于 2 5 5nm检测。结果 :线性范围为 0 0 4～ 12 μg·mL-1,萃取回收率为 85 2 %～ 89 9% ,加样回收率为 97 0 %～ 10 0 1% ,日内RSD为 0 4 5 %～ 2 72 % ,日间RSD为 1 3 1%～ 4 4 0 %。结论 :本法简便 ,准确 ,重现性好 ,用于测定 12名健康志愿者口服酮洛芬胶囊后血药浓度取得良好结果 Objective: To determine the concentration of ketoprofen in human plasma by high performance liquid chromatography. METHODS: Ketoprofen and naproxen (internal standard) were extracted from plasma samples with petroleum ether-diethyl ether (1: 1), and were separated on a Spherisorb C18 column with methanol-50 mmol·L-1 sodium phosphate monobasic 7) as the mobile phase separation, detected at 2545nm. Results: The linear range was 0 0 4 ~ 12 μg · mL-1, the extraction recovery was 85 2% -89 9%, the recovery was 97 0% -1 0 0 1%, and the intra-day RSD was 0 45% 2 72%, and the daytime RSD was 133% -440%. Conclusion: This method is simple, accurate and reproducible. It was used to determine the plasma concentration of ketoprofen in 12 healthy volunteers and achieved good results