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目的探讨叶黄素对老年痴呆(AD)模型小鼠学习记忆能力的影响及相关作用机制。方法采用5月龄雄性快速老化痴呆模型小鼠(SAMP8)作为自然发病的AD模型,随机分为AD模型组和3个叶黄素剂量组(15、30、60 mg/kg BW),采用正常老化小鼠(SAMR1)作为正常对照组,连续灌胃8周。灌胃结束后采用Morris水迷宫对小鼠进行学习记忆能力测验,取小鼠脑组织检测超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽(GSH)、丙二醛(MDA)和乙酰胆碱酯酶(Ach E)水平,取小鼠海马组织进行病理学观察。结果与AD模型组比较,叶黄素中、高剂量组的潜伏期明显缩短,差异有统计学意义(P<0.05);3个叶黄素剂量组的CAT、Ach E活性均明显升高,GSH含量明显增加,MDA含量明显降低,差异均有统计学意义(P<0.05);叶黄素高剂量组海马组织细胞异常明显减少。结论叶黄素可改善快速老化痴呆模型小鼠的学习记忆能力,其作用机制之一可能是提升小鼠的抗氧化能力,减少氧化应激损伤。
Objective To investigate the effects of lutein on learning and memory abilities of Alzheimer’s disease (AD) model mice and its related mechanism. Methods A 5-month old male model of accelerated aging dementia (SAMP8) was used as a natural AD model and randomly divided into AD model group and three lutein dose groups (15, 30 and 60 mg / kg BW) Aged mice (SAMR1) served as normal control group and were given continuous gavage for 8 weeks. At the end of gavage, Morris water maze was used to test the learning and memory ability of mice. The contents of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) Aldehyde (MDA) and acetylcholinesterase (Ach E) were measured in the hippocampus of mice. Results Compared with AD model group, the latency of lutein middle and high dose group was significantly shortened, the difference was statistically significant (P <0.05); CAT, Ach E activity of three lutein dose groups were significantly increased, GSH content was significantly (P <0.05). The abnormal high level of lutein in hippocampus tissue cells significantly reduced. Conclusion Lutein can improve the learning and memory abilities of mice with rapid aging dementia. One of the mechanisms may be to enhance the antioxidant capacity and reduce the oxidative stress in mice.