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作者用的是Wistar雄性大鼠,体重200-220克,分成实验组与对照组,每组6只。实验组:给予甲基汞2mg/kg,灌胃,自由进食喂养11天。对照组:给予生理盐水2mg/kg。用自动记录分光光度计在30℃恒温条件下分别检测了大鼠肾和尿中碱性磷酸酶、乳酸脱氢酶、溶菌酶、谷氨酸脱氢酶和酸性磷酸酶的活性,并测定了肾总汞量。 结果:表1为肾内汞量和酶活性的改变。实验组:肾汞量为62±9μg/g(组织湿重),肾内碱性磷酸酶、乳酸脱氢酶和溶菌酶的活性受抑,而酸性磷酸酶活性增加,谷氨酸脱氢酶活性不变。表2为尿中酶活性的改变。实验组尿中酶活性增加,碱性
The authors used Wistar male rats, weighing 200-220 g, divided into experimental and control groups, 6 in each. Experimental group: given methylmercury 2mg / kg, gavage, free feeding for 11 days. Control group: given saline 2mg / kg. The activities of alkaline phosphatase, lactate dehydrogenase, lysozyme, glutamate dehydrogenase and acid phosphatase in kidney and urine of rats were respectively detected by automatic recording spectrophotometer at a constant temperature of 30 ° C, Total mercury in the kidney. Results: Table 1 shows changes in the amount of mercury in the kidney and enzyme activity. In the experimental group, the amount of mercury in the kidney was 62 ± 9 μg / g (wet weight of the tissue), the activities of renal alkaline phosphatase, lactate dehydrogenase and lysozyme were inhibited while the activity of acid phosphatase was increased. The activity of glutamate dehydrogenase Activity unchanged. Table 2 changes in urinary enzyme activity. Urine enzyme activity increased, alkaline