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分布在寒冷地带的落叶木本植物,秋季到冬季间形成了被鳞片包被的冬眠芽,以抵御寒冷和为萌发作好准备。我们利用这一自然力,开发了在液态N中保存贵重遗传资源的方法。获得带有小段枝条的抗冻性冬眠芽,将其从0℃开始慢慢地降到-20℃或-30℃,使芽组织内的水分含量减少,这样,冬眠芽就能忍耐液态N的超低温。贮存后再复原个体的程序首先是解冻。对桑而言,须在5分钟内急速地使温度升到37℃,而对于梨,则要在3～4小时内慢慢地升温到0℃。然后,将冬芽的顶端摘下进行培养,可获得再生个体。目前,用液态N保存桑,保存期约一年。
Deciduous woody plants, distributed in the cold, form a scaled dormant bud between fall and winter to resist the cold and prepare for germination. We use this natural force to develop ways to conserve valuable genetic resources in liquid N. Frost-tolerant dormant buds with short shoots were obtained and slowly dropped from 0 ° C to -20 ° C or -30 ° C to reduce the moisture content in the bud tissue so that the hibernating buds were able to tolerate liquid N Ultra-low temperature. The procedure of restoring the individual after storage is first thawing. For mulberry, the temperature must be rapidly raised to 37 ° C in 5 minutes, and slowly to 0 ° C in 3 to 4 hours for pear. Then, the tip of the winter bud is removed and cultured to obtain a regenerated individual. At present, the preservation of liquid N with mulberry, storage period of about one year.