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目的:制备多壁碳纳米管(MWNT)修饰玻碳电极,并研究灯盏花素在该电极上的电化学行为及其测定方法。方法:循环伏安法和差示脉冲伏安法。结果:与裸玻碳电极(GCE)相比,MWNT膜修饰电极能显著提高灯盏花素的氧化峰电流。在pH=7.0的磷酸缓冲溶液中,灯盏花素在MWNT/GCE电极上出现1组氧化还原峰,Epa=0.19V,Epc=0.07V。灯盏花素氧化峰电流与其浓度在2×10-7～6×10-5mol·L-1范围内呈线性关系,检出限为2×10-7mol·L-1。灯盏花素样品进行5次平行测定的RSD为0.37%,平均回收率为99.5%。结论:MWNT膜对灯盏花素的电化学氧化有明显的催化作用,本法是一种可靠、快捷、灵敏的检测方法,可以用于灯盏花素含量的测定。
OBJECTIVE: To prepare multi-walled carbon nanotube (MWNT) modified glassy carbon electrode, and to study the electrochemical behavior and determination method of scutellarin on this electrode. Methods: Cyclic voltammetry and differential pulse voltammetry. Results: Compared with bare glassy carbon electrode (GCE), MWNT film modified electrode can significantly increase the oxidation peak current of breviscapine. In the phosphate buffer solution of pH=7.0, a redox peak appeared on the MWNT/GCE electrode of breviscapine with Epa=0.19V and Epc=0.07V. The linear relationship between breviscapine oxidation peak current and its concentration in the range of 2×10-7～6×10-5mol·L-1, the detection limit was 2×10-7mol·L-1. The RSD of five parallel determinations of breviscapine samples was 0.37% and the average recovery was 99.5%. Conclusion: MWNT membrane has obvious catalytic effect on the electrochemical oxidation of breviscapine. This method is a reliable, rapid and sensitive detection method and can be used for the determination of breviscapine content.