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目的体外扩增经ＣＤ３单抗（ＣＤ３ＭｃＡｂ）活化的杀伤细胞（ＣＤ３ＡＫ），并测定其抗肿瘤细胞作用和表型变化。②方法取１３例肺癌和２例肝癌病人外周血，经密度梯度离心获取淋巴细胞，以ＣＤ３ＭｃＡｂ和ＩＬ－２作为诱导剂，采用液相三步扩增方法扩增培养，并分别用ＭＴＴ和ＩＦＡ法与自体ＬＡＫ对照细胞比较细胞动力学、细胞毒作用和表型变化。③结果ＣＤ３ＡＫ细胞扩增速度、数量和抗肿瘤活性均优于自体ＬＡＫ细胞，其表型为ＣＤ３，ＣＤ４，ＣＤ８和ＣＤ１６的特殊异源淋巴细胞群。其中ＣＤ４亚群表型数量随培养时间延长明显升高（χ２＝７．６６，Ｐ＜０．０１），而ＣＤ１６则明显下降（χ２＝５．２３，Ｐ＜０．０５），该亚群表型变化可能与培养时间有关。④结论ＣＤ３ＡＫ细胞具有较强的杀肿瘤细胞作用，同时也可作为基因治疗的理想载体细胞。
Objective To amplify CD3 monoclonal antibody (CD3AK) activated CD3AK in vitro and determine its antitumor effect and phenotypic changes. 2 Methods Peripheral blood of 13 patients with lung cancer and 2 patients with liver cancer were collected. The lymphocytes were obtained by density gradient centrifugation. CD3 McAb and IL-2 were used as inducers. The three-step liquid phase amplification method was used to amplify the cultured cells. MTT and IFA were used respectively. Methods Cell viability, cytotoxicity and phenotypic changes were compared with autologous LAK control cells. 3 Results The proliferation rate, number, and anti-tumor activity of CD3AK cells were better than that of autologous LAK cells. The phenotype of CD3AK cells was a special heterologous lymphocyte population of CD3, CD4, CD8, and CD16. The number of phenotypes of CD4 subpopulations increased significantly with the prolongation of culture time (χ2=7.66, P<0.01), while CD16 significantly decreased (χ2=5.23, P<0.05). Phenotypic changes may be related to the culture time. 4 Conclusion CD3AK cells have a strong role in the killing of tumor cells, but also can be used as ideal carrier cells for gene therapy.