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[目的]研究罗布麻组织培养快繁技术,为其产业化栽培提供种苗来源。[方法]通过处理罗布麻种子获得无菌苗,切取无菌苗子叶、胚轴和茎尖置于不同激素配比的培养基上,比较不同激素浓度组合对子叶和胚轴分化、茎尖快繁、再生芽快繁和快繁苗生根的影响。[结果]子叶和胚轴分化形成再生芽的最佳培养基分别为MS+2.0mg/LBA+0.03mg/LNAA和MS+0.07mg/LNAA;MS+2.0mg/LBA+0.02mg/LNAA为茎尖快繁的最佳培养基;MS+1.9mg/LBA+1.7mg/LNAA为再生芽快繁的最佳培养基;1/2MS+0.6mg/LNAA为快繁苗的最佳生根培养基。[结论]该研究筛选出了罗布麻组培快繁技术体系的培养基激素配比,为罗布麻产业化栽培提供了技术保障。
[Objective] The research aimed to study the rapid propagating technology of Apocynum venetum tissue and provide seed source for its industrialization cultivation. [Method] The sterile seedlings were obtained by treating the seeds of Apocynum venetum L., the sterile seedling leaves were cut off, the hypocotyls and stem tips were placed on the medium with different hormone ratios, and the cotyledon and hypocotyls were differentiated with different combinations of hormone concentrations. Effects of Regeneration, Regeneration of Buds on Rapid Propagation and Rooting of Rapid Propagation Seedlings. [Result] The optimal culture medium for cotyledon and hypocotyls to form regenerated shoots was MS + 2.0mg / LBA + 0.03mg / LNAA and MS + 0.07mg / LNAA, respectively. MS + 2.0mg / LBA + 0.02mg / The best medium for fast propagation was MS + 1.9mg / LBA + 1.7mg / LNAA. The best medium for regeneration was 1 / 2MS + 0.6mg / LNAA. [Conclusion] This research screened the hormone and hormone ratio of Apocynum venetum Tissue culture technique system, which provided the technical support for the industrialization cultivation of Apocynum venetum.