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本文用ANS和DPH为荧光探剂,研究苯巴比妥(PB)诱导下大鼠肝微粒体膜脂区流动性与膜药酶活性变化的相关性。结果表明,经PB诱导后在增加肝微粒体蛋白质含量,P-450含量及NADPH-细胞色素C还原酶等酶活性的同时,肝微粒体膜流动性明显增大,且膜深层流动性的增大与膜氨基比林N-脱甲基酶、细胞色素C还原酶活性增加有明显的直线正相关。膜胆固醇/碑脂比值明显降低。此结果提示,肝微粒体膜流动性的适当增大与PB增加单胺氧化酶系统活性之间可能存在着某种联系。
In this paper, ANS and DPH as fluorescent probes to study the phenobarbital (PB) induced rat liver microsomal membrane lipid fluid mobility and membrane enzyme activity changes. The results showed that the fluidity of the liver microsomal membrane was significantly increased with the increase of protein content and P-450 content of liver microsomes and enzyme activity of NADPH-cytochrome C reductase induced by PB, and the increase of membrane depth fluidity Large and membrane aminopyrine N-demethylase, cytochrome C reductase activity increased significantly positive correlation. Membrane cholesterol / steatite ratio was significantly lower. This result suggests that there may be some connection between the appropriate increase of fluidity of the liver microsomal membrane and the increase of monoamine oxidase system activity by PB.