以Ｄ．Ｓ．Ｄ．酸为桥基，氯三嗪为活性基，对－氨基苯甲酸、对－氨基苄基膦酸、对－氨基苯磺酸为尾基合成了三只对称型无色活性配基，其结构经波谱法及色谱法验证；比较了它们对碱性磷酸酶的纯化功能。结果表明：以苯甲酸及苄基膦酸作尾基者效果较好，回收率达８０％以上；配基浓度及缓冲液ｐＨ值对回收率影响明显。 D. S. D. Acid as the bridge group, chlorotriazine as the active group, p-aminobenzoic acid, p-aminobenzylphosphonic acid, p-aminobenzenesulfonic acid as the tail group synthesized three symmetrical colorless active ligand, the structure of the Spectroscopy and chromatographic validation; compared their purification of alkaline phosphatase function. The results showed that benzoic acid and benzylphosphonic acid as the tail end of the better, the recovery rate of 80% or more; Ligand concentration and pH value of the buffer significantly affected the recovery.