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设计合成 3种含芘荧光化学敏感器———化合物 (Ⅰ ) ,(Ⅱ ) ,(Ⅲ ) .研究了芘丁酸 ,化合物 (Ⅰ ) ,(Ⅱ ) ,(Ⅲ )等与腺嘌呤 ,5 腺苷单磷酸二钠盐 (AMP) ,5 腺苷二磷酸二钠盐 (ADP) ,5 腺苷三磷酸四钠盐 (ATP) ,5 脱氧胸腺嘧啶三磷酸四钠盐 (dTTP)等生物活性分子相互作用时的荧光猝灭光谱 ,运用Stern Volmer猝灭等方程分别求出它们相互作用时的猝灭常数及稳定常数 .结合化合物荧光寿命的测定 ,对含芘荧光化学敏感器在不同波长激发下所得的荧光光谱以及基态下自由分子的CPK模型等对上述含芘荧光化学敏感器同核苷磷酸盐的作用机制进行了讨论 .
Three kinds of pyrene fluorescent chemical sensors - compounds (Ⅰ), (Ⅱ) and (Ⅲ) were designed and synthesized.The effects of pyrene butyric acid, compounds Ⅰ, Ⅱ and Ⅲ with adenine, Adenosine monophosphate disodium salt (AMP), 5 adenosine diphosphate disodium salt (ADP), 5 adenosine triphosphate tetrasodium salt (ATP), 5 thymidine pyrimidine tetrasodium salt (dTTP) and other biological activity Molecular quenching spectroscopy and the Stern Volmer quenching equations were used to determine the quenching constants and the stability constants of these interactions respectively.According to the determination of the fluorescence lifetime of the compounds, the fluorescence excitation of pyrene-containing fluorescent chemical sensors at different wavelengths The fluorescence spectra obtained under this mode and the free radical CPK model in the ground state are discussed in detail.