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本文报道小鼠睾丸早期精细胞的微核检测方法.以2 mg/kg丝裂霉素C腹腔注射小鼠,于给药后13、14和15天杀鼠,用胶原酶和透明质酸酶分离睾丸细胞并制片,测得徽核率分别为3.5‰、4.75‰和2.50‰,与对照组的0.6‰比较差异显著,表明对依赖于S期的化学物的染色体损伤作用,于给药后第14天杀鼠制片,检测早期精细胞微核是一种理想的简易检测雄性生殖细胞染色体损伤的方法.
This paper reports the micronucleus test of early testicular spermatogenic cells in mice.Mice were injected intraperitoneally with 2 mg / kg mitomycin C and killed on the 13th, 14th and 15th day after administration, and the collagenase and hyaluronidase Separation of testicular cells and preparation, the measured rates were 3.5 ‰, 4.75 ‰ and 2.50 ‰, respectively, compared with 0.6 ‰ in the control group, showing significant differences in the chromosomal damage to chemicals dependent on the S phase, Fourteen days after killing rats films, detection of early micronuclei of sperm cells is an ideal simple method to detect male germ cell chromosome damage.