论文部分内容阅读
目的观察和分析血管紧张素Ⅱ(AngⅡ)对人子宫内膜上皮细胞(EECs)增殖和活力的影响。方法分离原代EECs并常规培养。试验分为对照组和AngⅡ组。对照组:加入不含血清的培养液;AngⅡ组:加入不同浓度的AngⅡ,通过噻唑蓝(MTT)染色察看各组细胞的增殖率;用ELISA法分析各组孵育液中Ⅰ型胶原(ColⅠ)和纤粘连蛋白(FN)的含量。免疫细胞化学技术分析各组EECs胞质内的α-平滑肌肌动蛋白(α-SMA)和上皮性钙粘附素(E-cadherin)的表达水平。结果不同浓度AngⅡ组的EECs增殖率均显著升高,且表现为剂量依赖的趋势;细胞内α-SMA含量(71.71±1.47)升高,E-cadherin含量(25.32±1.43)降低;ColⅠ和FN分泌量(0.774 7±0.048 0;0.758 0±0.042 0)均增加,与对照组比较,其差异有统计学意义(P<0.05)。结论 AngⅡ能使子宫内膜上皮细胞增殖,并刺激使其发生活力改变。
Objective To observe and analyze the effect of angiotensin Ⅱ on the proliferation and viability of human endometrial epithelial cells (EECs). Methods Primary EECs were isolated and cultured routinely. The experiment was divided into control group and Ang Ⅱ group. Control group: serum-free culture medium was added; AngⅡgroup: different concentrations of AngⅡ were added, the proliferation rate of each group was observed by MTT staining; type Ⅰcollagen (ColⅠ) And fibronectin (FN) content. Immunocytochemistry was used to analyze the expression of α-smooth muscle actin (α-SMA) and E-cadherin in the cytoplasm of EECs in each group. Results The proliferation rate of EECs in different concentrations of AngⅡgroup was significantly increased, and showed a dose-dependent trend. The intracellular α-SMA content (71.71 ± 1.47) and E-cadherin content (25.32 ± 1.43) (0.774 7 ± 0.048 0; 0.758 0 ± 0.042 0) increased, compared with the control group, the difference was statistically significant (P <0.05). Conclusion Ang Ⅱ can proliferate the endometrial epithelial cells and stimulate their vitality changes.