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目的探讨姜黄素对肝癌细胞株HepG2对顺铂敏感性的影响及其作用机制。方法采用不同浓度姜黄素(0、2.5、5、7.5、10μM)及顺铂(10、20、40、80μM)单用或联用处理HepG2细胞株后,通过MTT检测细胞增殖抑制率,流式细胞术检测细胞凋亡情况,Real-time PCR技术检测Survivin、Cyto-C、Bcl2、Bax的基因表达情况;western blot检测Survivin、Cyto-C、cleaved caspase-3、Bcl-2、Bax的蛋白表达。结果姜黄素以浓度依赖性方式抑制HepG2细胞株Survivin的m RNA表达和蛋白表达(P<0.05);姜黄素联合顺铂处理的HepG2细胞增殖抑制率及细胞凋亡率较单用顺铂处理均明显增加(P<0.05);姜黄素联合顺铂可显著抑制Survivin的蛋白表达,降低Bcl-2/Bax比例,增加Cyto-C、cleaved caspase-3蛋白表达(P<0.05)。结论姜黄素可增加HepG2细胞对顺铂的敏感性,可能与其抑制Survivin的表达,降低Bcl-2/Bax比例,增加cleaved caspase-3、Cyto-C蛋白的表达有关。
Objective To investigate the effect of curcumin on the sensitivity of hepatocellular carcinoma cell line HepG2 to cisplatin and its mechanism of action. Methods HepG2 cell lines were treated with different concentrations of curcumin (0, 2.5, 5, 7.5, and 10 μM) and cisplatin (10, 20, 40, and 80 μM) alone or in combination. The cell proliferation inhibition rate was measured by MTT. The cell apoptosis was detected by cell culture. The expression of Survivin, Cyto-C, Bcl2, and Bax genes was detected by Real-time PCR. The protein expression of survivin, Cyto-C, cleaved caspase-3, Bcl-2, and Bax were detected by western blot. . Results Curcumin inhibited the expression of Survivin mRNA and protein in HepG2 cell line in a concentration-dependent manner (P<0.05). The proliferation inhibition rate and apoptosis rate of curcumin and cisplatin-treated HepG2 cells were higher than those of cisplatin alone. Significantly increased (P <0.05); curcumin combined with cisplatin can significantly inhibit the expression of Survivin protein, reduce the ratio of Bcl-2/Bax and increase the expression of Cyto-C and cleaved caspase-3 protein (P <0.05). Conclusion Curcumin can increase the sensitivity of HepG2 cells to cisplatin, which may be related to its inhibition of Survivin expression, decrease of Bcl-2/Bax ratio and increase of cleaved caspase-3 and Cyto-C protein expression.