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在离体和活体条件下测定了多菌灵、三环唑对大丽轮枝菌的微菌核黑色素形成的影响,以及经2种杀菌剂处理产生的形态变异菌株对棉苗的致病力。结果表明:多菌灵在培养基内含量超过0.1μg/ml时,即可抑制大丽轮枝菌微菌核的形成,随着培养基内多菌灵浓度的增加,微菌核形成时间逐渐延长,形成量逐渐减少;三环唑浓度为0.5μg/ml时,可抑制微菌核的黑色素形成,微菌核从黑色变为浅红至红褐色。三环唑对微菌核黑色素形成呈可逆抑制,变色的微菌核菌落移入不含药的培养基后,大多可恢复形成黑色素。培养基内三环唑浓度的提高,也可抑制大丽轮枝菌微菌核的形成;因2种杀菌剂的抑制而丧失形成微菌核的白色菌丝体移入不含药的培养基,微菌核形成能力也不能恢复。多菌灵和三环唑经棉株吸收后均能抑制植株内大丽轮枝菌微菌核的形成,但对微菌核色素的形成未见有明显影响。2种杀菌剂处理产生的形态变异菌株的致病力与野生型菌株致病力差异不显著。
The effects of carbendazim and tricyclazole on the formation of Mycobacterium dahliae were analyzed in vitro and in vivo, and the virulence of morphological mutants treated with two fungicides against cotton seedlings . The results showed that carbendazim could inhibit the formation of M. sclerotiorum when the content of carbendazim in medium exceeded 0.1 μg / ml. With the increase of carbendazim concentration in culture medium, Gradually increased, and the amount of formation gradually decreased. When the concentration of tricyclazole was 0.5 μg / ml, the formation of melanin was inhibited, and the microscopic nucleus changed from black to light red to reddish brown. Tricyclazole showed a reversible inhibition on the formation of melanocytes. After the discoloration of the bacterial colonies moved into the drug-free medium, most of the melanosomes recovered melanin. The increase of the concentration of tricyclazole in the medium also inhibited the formation of Mycological Core of V. dahurica; the white mycelium which lost the formation of mycobacteria due to the inhibition of the two kinds of fungicides was transferred into the medium without drug, Mycosis can not be restored. Carbendazim and tricyclazole can inhibit the formation of Mycological nuclei in Verticillium dahliae after being absorbed by cotton plants, but no significant effect on the formation of Mycotoxin. The pathogenicity of the morphological variants produced by the two fungicides was not significantly different from that of the wild type.