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目的探讨落新妇甙对小鼠心脏移植后移植物血管病的抑制作用。方法建立小鼠颈部心脏移植模型,将受鼠随机分为环孢素A(CsA)组(术后每天用CsA5mg/kg灌胃)和落新妇甙组(术后每天用落新妇甙5mg/kg灌胃)。术后第60d切取移植心,观察移植心脏组织学变化,以HPIAS-1000图像分析仪测量冠状动脉管壁厚度与动脉直径的比值,根据平均内膜厚度和管腔狭窄程度对CAV病变进行评分。结果CsA组移植心脏可见明显的纤维化,大量单核细胞浸润,动脉硬化明显,冠状动脉管壁厚度与血管直径的比值为0.63±0.20,明显高于落新妇甙组的0.22±0.04(P<0.01);CsA组心脏移植物血管病变评分为(2.3±0.6)分,高于落新妇甙组的(1.1±0.3)分(P<0.05)。落新妇甙组心脏移植物的冠状动脉内膜病变轻微,内皮和内弹力层基本保持完整,平滑肌细胞增殖不明显。结论落新妇甙能减缓移植心冠状动脉硬化,对小鼠心脏移植后移植物血管病有明显的抑制作用。
Objective To investigate the inhibitory effect of Astilbin on the graft vascular disease after heart transplantation in mice. METHODS: A mouse model of cervical heart transplantation was established. Rats were randomly divided into the cyclosporine A (CsA) group (after daily gastric perfusion with CsA 5 mg/kg) and the astilbin women group (after surgery, 5 mg/day of the new woman was used). Kg gavage). On the 60th day after operation, the transplanted heart was harvested and the histological changes of the transplanted hearts were observed. The ratio of coronary artery wall thickness to arterial diameter was measured with the HPIAS-1000 image analyzer, and the CAV lesions were scored according to the mean intimal thickness and lumen stenosis. Results In the CsA group, there was obvious fibrosis in the transplanted heart, a large number of mononuclear cell infiltration, arteriosclerosis, and the ratio of coronary artery wall thickness to vessel diameter was 0.63±0.20, which was significantly higher than 0.22±0.04 (P<0.05). 0.01); CsA group heart graft vascular lesion score was (2.3 ± 0.6) points, higher than the astilbe women’s group (1.1 ± 0.3) points (P <0.05). The ascending heart disease of the ascending heart and graft group was mild, and the endothelium and inner elastic layer remained intact. The proliferation of smooth muscle cells was not obvious. Conclusion Astilbinate can slow down the coronary arteriosclerosis in the transplanted heart, and has a significant inhibitory effect on graft vascular disease after heart transplantation in mice.