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目的:观察青蒿琥酯对人白血病K562、K562/ADM细胞凋亡以及对p65表达的影响。方法:采用流式细胞仪检测青蒿琥酯在不同浓度和不同时间段对K562、K562/ADM细胞凋亡的影响,采用Western blot法检测15μmol·L-1青蒿琥酯在不同时间对K562细胞NF-κB p65表达的影响。结果:青蒿琥酯对K562细胞凋亡影响不明显,但对阿霉素耐药K562/ADM细胞影响较大,青蒿琥浓度为7.5μmol·L-1和15μmol·L-1时,K562/ADM细胞的调亡率明显高于K562细胞(P<0.01),15μmol·L-1青蒿琥酯作用后4 h后,K562/ADM细胞的调亡率明显增加(P<0.01);经15μmol·L-1青蒿琥酯作用后,p65表达随时间的增加而明显降低(P<0.01)。结论:青蒿琥酯通过下调P65的表达而诱导白血病细胞凋亡。
Objective: To observe the effect of artesunate on the apoptosis and the expression of p65 in human leukemia K562 and K562 / ADM cells. Methods: The effects of artesunate on the apoptosis of K562 and K562 / ADM cells were detected by flow cytometry. K562 and K562 / ADM cells were treated with 15μmol·L-1 artesunate at different times by Western blot. Effect of NF-κB p65 expression on cells. Results: Artesunate had no obvious effect on apoptosis of K562 cells, but had a significant effect on doxorubicin-resistant K562 / ADM cells. When artesunate concentration was 7.5μmol·L-1 and 15μmol·L-1, K562 / ADM cells was significantly higher than that of K562 cells (P <0.01). The apoptosis rate of K562 / ADM cells increased significantly after 4 h treatment with 15 μmol·L -1 artesunate (P <0.01) After treated with 15μmol·L-1 artesunate, the expression of p65 was significantly decreased with the increase of time (P <0.01). Conclusion: Artesunate can induce the apoptosis of leukemic cells by down-regulating the expression of P65.