脑啡肽自1975年发现以来,被认为是一种神经递质或神经调制物,与多种生理功能有关,因而受到高度重视和广泛研究。关于脑啡肽的放射免疫测定(RIA),国内外已有报导。自1981年以来,本实验室参照以往的报导,免疫家兔获得特异性较高的亮-脑啡肽(LEK)和甲硫-脑啡肽(MEK)抗血清,并改进了~(125)I-脑啡肽的制备方法,建立了相应的RIA。将此法应用于刺镇痛原理的研究,已取得初步成果,现将方法部分报告如下。 Since it was discovered in 1975, enkephalin is considered as a neurotransmitter or neuromodulator, which is related to various physiological functions. Therefore, enkephalin is highly valued and extensively studied. Radioimmunoassay (RIA) for enkephalin has been reported both at home and abroad. Since 1981, our laboratory has referred to the previous reports that the rabbits were immunized to obtain LEK and MEK antisera with high specificity, I-enkephalin preparation method, the establishment of the corresponding RIA. This method is applied to study the principle of analgesia, has achieved initial results, the method part of the report is as follows.