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目的:通过测定神经生长因子(Nerve growth factor,NGF)及其受体P75在早期自然流产和正常早孕绒毛组织中的表达情况,探讨其临床意义。方法:收集自然流产病例30例作为研究组和正常早孕人工流产病例30例作为对照组,采用两步法原位杂交免疫组化方法检测NGF及其受体P75在早期自然流产绒毛组织与正常早孕绒毛组织中的表达差异。结果:P75在绒毛组织中的染色阳性标本平均染色分值研究组(4.03±2.220)高于对照组(2.33±1.882),两组间比较差异有统计学意义(P<0.05);NGF在绒毛组织中的染色阳性标本平均染色分值研究组(2.83±2.019)低于对照组(4.30±2.200),两组间平均染色分值比较差异有统计学意义(P<0.05)。结论:NGF及其受体P75在自然流产绒毛组织中异常表达可能与自然流产的发病机理有关。
OBJECTIVE: To investigate the clinical significance of Nerve growth factor (NGF) and its receptor P75 in early spontaneous abortion and normal early pregnancy chorionic villi. Methods: Thirty cases of spontaneous abortion were collected as research group and 30 cases of normal pregnancy induced abortion as control group. Two-step in situ hybridization immunohistochemistry was used to detect the expression of NGF and its receptor P75 in early spontaneous abortion with normal early pregnancy Differences in expression in villous tissue. Results: The average staining score of P75 in villus tissue was significantly higher in study group (4.03 ± 2.220) than that in control group (2.33 ± 1.882) (P <0.05) The average staining score of the positive staining specimens in the study group (2.83 ± 2.019) was lower than that of the control group (4.30 ± 2.200), and the difference was statistically significant (P <0.05). Conclusion: Abnormal expression of NGF and its receptor P75 in spontaneous abortion chorion may be related to the pathogenesis of spontaneous abortion.