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背景:载脂蛋白E基因敲除小鼠形成的动脉粥样硬化病变与人类全身动脉粥样硬化好发处相近,是目前建立动脉粥样硬化理想的动物模型。目的:研究载脂蛋白E基因敲除小鼠不同周龄动脉粥样硬化的病理进程,探讨不同饮食对载脂蛋白E基因敲除小鼠动脉粥样硬化发生发展的影响。方法:将8周龄雄性载脂蛋白E基因敲除小鼠,随机分为2组,分别给予高脂饮食和普通饮食喂养8,12,16,20,24周。结果与结论:血清学指标检测显示,不同周龄的高脂饮食组血清中总胆固醇、三酰甘油和低密度脂蛋白胆固醇水平显著高于普通饮食组(P<0.05),呈时间依赖性。大体和冰冻切片油红O染色结果显示,高脂饮食组动脉粥样硬化管腔斑块面积显著高于普通饮食组(P<0.05),呈时间依赖性,此时两组各周龄小鼠管腔斑块面积相比均有显著性意义(P<0.05),小鼠在高脂饮食16周时主动脉可见明显的脂质斑块。结果表明,实验成功构建了载脂蛋白E基因敲除动脉粥样硬化模型小鼠,此模型形成脂质条纹和纤维增生病变的时间较普通饮食组更快。
BACKGROUND: Atherosclerotic lesions formed by apolipoprotein E knockout mice are similar to those occurring in human whole body atherosclerosis and are ideal animal models for establishing atherosclerosis. OBJECTIVE: To study the pathological progression of atherosclerosis in different weeks of apolipoprotein E gene knockout mice and to explore the effects of different diets on the development of atherosclerosis in apolipoprotein E knockout mice. Methods: 8-week-old male apolipoprotein E knockout mice were randomly divided into two groups, fed with high-fat diet and normal diet respectively for 8, 12, 16, 20, and 24 weeks. RESULTS AND CONCLUSION: The serum levels of total cholesterol, triglyceride and low density lipoprotein cholesterol in serum of high-fat diet group were significantly higher than those of normal diet group (P <0.05) in a time-dependent manner. The results of oil red O staining showed that the plaque area of atherosclerosis in high-fat diet group was significantly higher than that in normal diet group (P <0.05), and was time-dependent. At this time, (P <0.05). The mice showed obvious lipid plaques in the aorta at 16 weeks of high-fat diet. The results showed that the model of atherosclerosis in apolipoprotein E gene knockout mice was constructed successfully. The time for the formation of lipid streaks and fibrotic lesions was faster in this model than that in the normal diet group.