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目的:探讨解淀粉芽孢杆菌或解淀粉芽孢杆菌培养液(代表细菌产物)是否导致BALB/c小鼠哮喘。方法:40只SPF级雌性BALB/c小鼠随机分为4组(每组10只),即对照组、卵清蛋白(OVA)组、培养液组、细菌组。于第0天、第7天用OVA小鼠腹腔注射致敏,从第14天到第28天用OVA液对小鼠进行雾化吸入激发,1次/d,每次30 min;对照组、培养液组、细菌组分别用生理盐水、细菌培养液、细菌进行致敏和激发试验。结果:实验表明OVA组和细菌组两组的活体肺功能(APTI)值均高于对照组;与对照组相比较,OVA组、细菌组两组的管壁厚度、肺泡灌洗液中白细胞总数、嗜酸性粒细胞百分比均有显著升高(P<0.01),而淋巴细胞百分比均显著降低,尤其是细菌组更加突出;与对照组比较,OVA组和细菌组的血清中IgE浓度均为显著升高(P<0.01)。结论:用解淀粉芽孢杆菌建立了BALB/c小鼠哮喘动物模型,并证明导致小鼠哮喘的主要因子是细菌。
Objective: To investigate whether Bacillus amyloliquefaciens or Bacillus amyloliquefaciens culture medium (representative of bacterial products) lead to asthma in BALB / c mice. Methods: Forty SPF female BALB / c mice were randomly divided into 4 groups (10 in each group): control group, ovalbumin (OVA) group, culture medium group and bacteria group. On day 0 and day 7, mice were sensitized intraperitoneally with OVA mice. Mice were challenged with OVA solution on day 14 to 28, once a day for 30 minutes. In the control group, Culture medium group and bacteria group were sensitized and challenged with saline, bacterial culture and bacteria respectively. Results: The experimental results showed that the APTI values in OVA group and bacterial group were higher than those in control group. Compared with control group, the thickness of wall, the total leukocyte count in BALF , The percentage of eosinophils were significantly increased (P <0.01), while the percentage of lymphocytes was significantly decreased, especially in the bacterial group more prominent; compared with the control group, OVA group and bacterial serum IgE concentrations were significant Increased (P <0.01). Conclusion: The animal model of asthma in BALB / c mice was established by Bacillus amyloliquefaciens and the main factor that induced asthma in mice was bacteria.