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[目的]观察葛花解酲汤对酒精性脂肪肝(AFL)大鼠生化指标和肝脏病理的影响。[方法]72只SD大鼠随机分为正常组,模型组,中药大、小剂量组和易善复组。用50%乙醇制备模型,正常组仅予蒸馏水10 ml/(kg.d),其余各组在造模同时,分别予201、0 g/(kg.d)中药浓缩液和91.2 mg/(kg.d)易善复混悬液灌胃,连续14周,处死,观察肝脏病理变化,测定血清生化指标、游离脂肪酸(FFA)及血浆和肝匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)水平。[结果]模型组与正常组比较,三酰甘油(TG)、FFA、MDA明显升高(P<0.01),而SOD、GSH-PX明显降低(P<0.01);中药组与模型组比较,TG、FFA、MDA明显下降(P<0.01),而SOD、GSH-PX明显升高(P<0.01);病理结果显示模型组肝细胞出现不同程度的脂肪变性和点、灶状坏死;中药大剂量组和易善复组脂肪变性和炎症程度较模型组明显减轻。[结论]葛花解酲汤对AFL有一定的防治作用。
[Objective] To observe the effect of Gehua Jieyu Decoction on biochemical indexes and hepatic pathology of alcoholic fatty liver (AFL) rats. [Methods] Seventy-two SD rats were randomly divided into normal group, model group, big and small doses of traditional Chinese medicine and Yi Shanfu group. The model was prepared with 50% ethanol. The normal group only received distilled water of 10 ml/(kg.d). In the rest of the groups at the same time, they were given 201,0 g/(kg.d) of Chinese herbal concentrate and 91.2 mg/(kg). .d) Ethanol suspension was intragastrically administered for 14 consecutive weeks. The liver pathological changes were observed. Serum biochemical parameters, free fatty acid (FFA) and malondialdehyde (MDA), superoxide in plasma and liver homogenate were measured. Dismutase (SOD) and glutathione peroxidase (GSH-PX) levels. [Results]Compared with the normal group, the triglyceride (TG), FFA, and MDA levels in the model group were significantly higher (P<0.01), while the SOD and GSH-PX were significantly lower (P<0.01); the Chinese medicine group was compared with the model group. TG, FFA and MDA were significantly decreased (P<0.01), while SOD and GSH-PX were significantly increased (P<0.01). The pathological results showed that the hepatocytes of the model group had varying degrees of steatosis and focal, focal necrosis; The degree of steatosis and inflammation in the dose group and Yishan Fu group was significantly reduced compared with the model group. [Conclusion] Gehua Jieyu Decoction can prevent and treat AFL.