目的 观察食管癌患者经肠外营养(PN)支持后辅助性T淋巴细胞所分泌细胞因子IFN-γ的变化,为PN作为一种免疫疗法提供依据。方法 应用酶联免疫吸附法检测食管癌患者体内IFN-γ的含量,同时检测外周血单个核细胞经或不经PHA刺激后培养上清液中的IFN-γ的含量。结果 经肠外营养支持后,体内IFN-γ的含量高于对照组,经PHA刺激后,两组间的这一差异更加明显。结论 围手术期肠外营养支持能够促进T淋巴细胞亚群的移动,提高食管癌患者的细胞免疫功能,有利于改善患者的预后。 Objective To observe the changes of cytokine IFN-γ secreted by helper T lymphocytes in patients with esophageal cancer after parenteral nutrition (PN) support, so as to provide basis for PN as an immunotherapy. Methods The content of IFN-γ in esophageal cancer patients was detected by enzyme-linked immunosorbent assay (ELISA), and the content of IFN-γ in supernatant of peripheral blood mononuclear cells was detected with or without PHA. Results After parenteral nutrition support, the content of IFN-γ in vivo was higher than that of the control group. After PHA stimulation, the difference between the two groups was more obvious. Conclusion Perioperative parenteral nutrition can promote the movement of T lymphocyte subsets and improve the cellular immune function in esophageal cancer patients, which is beneficial to improve the prognosis of patients.