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目的探讨细胞外信号调解激酶(ERK)信号转导通路在新生大鼠海马神经干细胞增殖中的作用。方法体外培养新生大鼠海马神经干细胞,传至第4代后进行单细胞克隆培养并传代。将培养细胞分为2组,对照组采用神经干细胞培养液进行培养,实验组中在神经干细胞培养液的基础上添加不同浓度的ERK通路抑制剂U0126,Western Blot检测加入细胞中ERK及P-ERK的表达情况,MTT法检测其对神经干细胞增殖的影响。结果神经干细胞加入U0126后ERK磷酸化水平明显低于对照组;当U0126浓度为10M时,细胞的增殖速度较对照组明显降低。结论神经干细胞的增殖可能与ERK信号转导通路有关。
Objective To investigate the role of extracellular signal-regulated kinase (ERK) signal transduction pathway in the proliferation of neural stem cells in neonatal rat hippocampus. Methods The hippocampal neural stem cells of neonatal rats were cultured in vitro. After passage 4, they were subcultured and passaged. The cultured cells were divided into two groups. The control group was cultured with neural stem cell culture medium. Different concentrations of ERK pathway inhibitor U0126 were added to the experimental group on the basis of neural stem cell culture medium. The expression of ERK and P-ERK The expression of neural stem cells was detected by MTT assay. Results After U0126 neural stem cells were added, the level of ERK phosphorylation was significantly lower than that of the control group. When the concentration of U0126 was 10M, the proliferation rate of cells was significantly lower than that of the control group. Conclusion The proliferation of neural stem cells may be related to ERK signal transduction pathway.