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荧光生物分析技术在科研及医疗机构已获得广泛应用.常规的荧光免疫分析方法由于采用传统生物探针(如荧光染料及量子点等)作为标记,易受到杂散光及生物组织自荧光的干扰.利用无机稀土纳米荧光探针优异的发光性能,如长荧光寿命的下转移发光、近红外激发的上转换发光以及无需激发源的长余辉发光,可有效解决背景荧光的干扰.本文从基础的物理化学性能到生物应用角度出发综述了无机稀土纳米发光材料的最新进展,包括材料的控制合成、表面功能化、光学性能及其在无背景荧光生物分析方面的应用示范,并对该类材料未来的发展趋势与努力的方向作了进一步的远景展望.
Fluorescence bioanalysis has been widely used in scientific research and medical institutions.Using traditional biological probes (such as fluorescent dyes and quantum dots) as the markers, conventional fluorescence immunoassay methods are susceptible to stray light and self-fluorescence of biological tissues. The luminescent properties of inorganic rare earth nanofluorescence probes, such as down-shift luminescence with long fluorescence lifetime, up-conversion luminescence with near-infrared excitation and long afterglow luminescence without excitation source, can effectively solve the background fluorescence interference. Recent advances in inorganic rare earth nano luminescent materials are reviewed, including the control of synthesis, surface functionalization, optical properties and their application in the background-free fluorescence bioanalysis. The future applications of these materials in the future Development trend and the direction of efforts made further prospects.