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从‘红星’苹果(Malus domestica ‘Delicious’)果实中克隆获得了MdMYB1基因,通过生物信息学分析(in silico analysis)和活体细胞融合蛋白荧光观察,确定其编码蛋白定位于细胞核。同时,克隆了MdDFR和MdUFGT基因的启动子,模拟分析表明2个基因的启动子序列中含有MYB结合的顺式作用元件。为了探讨MdMYB1转录因子是否调控MdDFR和MdUFGT基因的表达,对不同光照条件下果皮花青素含量和基因表达进行了检测,结果表明光照能够诱导花青素积累,并迅速启动3个基因表达,且3个基因表现出高度相似的表达模式,表明MdMYB1转录因子可能直接调控MdDFR和MdUFGT基因的表达。
The MdMYB1 gene was cloned from the fruit of ’Malus domestica’ Delicious’ and identified by in silico analysis and fluorescent observation of living cell fusion protein. At the same time, the promoters of MdDFR and MdUFGT genes were cloned, and the mimic analysis showed that the promoter sequences of two genes contained MYB-binding cis-acting elements. In order to investigate whether MdMYB1 transcription factor regulates the expression of MdDFR and MdUFGT genes, the anthocyanin content and gene expression in pericarp were detected under different light conditions. The results showed that light could induce the accumulation of anthocyanin and rapidly start the expression of three genes Three genes showed highly similar expression patterns, indicating that MdMYB1 transcription factor may directly regulate the expression of MdDFR and MdUFGT genes.