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三、组织解离法采用化学试剂处理被检材料,使组织中细胞间的胞间层溶解而使细胞分离,此法可以更完整而清楚地观察组织中的个别细胞的特征,如导管,纤维,石细胞等。组织解离所用的化学试剂可因材料的性质而异,常用以下三法: 1.氢氧化钾法适用于组织柔软的材料,如叶。取被检材料,切成小块(长、宽、厚各约3毫米以下),置试管或小烧杯中,加5%氢氧化钾溶液2～5ml,水溶加热20～30分钟,至用解剖刀轻压材料即能散开为度,倾去碱液,材料用水洗涤后取少量置载玻
Third, the dissociation method using chemical reagents to treat the test material, so that the intercellular cells in the tissue to dissolve the cells to separate, this method can be more complete and clear observation of the characteristics of individual cells in the organization, such as catheters, fiber , stone cells and so on. The chemical reagents used for tissue dissociation can vary depending on the nature of the material. The following three methods are commonly used: 1. The potassium hydroxide method is suitable for the organization of soft materials such as leaves. Take the test material, cut into small pieces (length, width, thickness of each about 3 mm or less), placed in a test tube or a small beaker, add 5% potassium hydroxide solution 2 ~ 5ml, water soluble heating 20 ~ 30 minutes, to use dissection The knife light pressure material can be dispersed into degrees, and the lye is poured. After the material is washed with water, a small amount of glass is placed