论文部分内容阅读
背景:鉴于野生水母雪莲濒临灭绝,军事医学科学院建立了一种人工培养水母雪莲的方法。目的:探讨水母雪莲细胞培养物对高脂大鼠的调血脂作用。设计:完全随机设计,对照实验研究。单位:由解放军总医院心内科和军事医学科学院生物工程研究所合作。材料:实验于2003-03/06在解放军总医院医学实验动物中心完成。实验动物由解放军总医院实验动物中心提供。雄性SD大鼠64只,体质量200～220g,随机分为4组,每组16只:正常对照组、高脂模型组、高剂量组和低剂量组。干预:正常对照组仅喂基础饲料,高脂模型组喂高脂饲料,高剂量组喂高脂饲料的同时饲以水母雪莲细胞培养物500mg/Kg,低剂量组喂高脂饲料的同时饲以水母雪莲细胞培养物50mg/Kg。给药1次/d,3周后采血,测定血脂水平及肝肾功能。主要观察指标:各组间血脂水平差异及肝肾功能变化。结果:高剂量组胆固醇、三酰甘油、低密度脂蛋白水平低于高脂模型组犤(2.66±0.51)mmol/L,(3.23±0.57)mmol/L,P=0.030;(1.59±0.77)mmol/L,(2.48±0.83)mmol/L,P=0.023;(1.68±0.31)mmol/L,(2.07±0.43)mmol/L,P=0.032犦。高剂量组高密度脂蛋白水平高于高脂模型组犤(0.98±0.29)mmol/L,(0.66±0.37)mmol/L,P=0.045犦。各组间肝肾功能差异无显著性意义(P>0.05)。结论:水母雪莲细胞培养物对
Background: In view of the extinction of the wild jellyfish Snow Lotus, the Academy of Military Medical Sciences has established a method for artificially cultivating the jellyfish jellyfish. Objective: To investigate the lipid-regulating effects of Saussurea sagittifolia cell culture on hyperlipidemic rats. Design: Completely random design, controlled experimental study. Unit: Coordinated by the Department of Cardiology, General Hospital of PLA, and the Institute of Bioengineering, Academy of Military Medical Sciences. MATERIALS: The experiment was performed at the Medical Experimental Animal Center of PLA General Hospital from March 2003 to March 2003. Experimental animals were provided by the Experimental Animal Center of the PLA General Hospital. 64 male SD rats, weighing 200-220 g, were randomly divided into 4 groups with 16 rats in each group: normal control group, high-fat model group, high-dose group, and low-dose group. Intervention: normal control group fed only basal diet, high-fat model group fed high-fat diet, high-dose group fed high-fat diet while feeding jellyfish Saussurea cell culture 500mg/Kg, while low-dose group fed high-fat diet while feeding Jellyfish Saussurea Cell Culture 50 mg/Kg. The administration was performed once/d, blood was collected after 3 weeks, and blood lipid levels and liver and kidney function were measured. MAIN OUTCOME MEASURES: Differences in blood lipid levels and changes in liver and kidney function among the groups. Results: The levels of cholesterol, triacylglycerol and LDL in the high-dose group were lower than those in the high-fat model group (2.66±0.51) mmol/L, (3.23±0.57) mmol/L, P=0.030; (1.59±0.77) Mmol/L, (2.48±0.83) mmol/L, P=0.023; (1.68±0.31) mmol/L, (2.07±0.43) mmol/L, P=0.032犦. The high-dose lipoprotein level in the high-dose group was higher than that in the high-fat model group (0.98±0.29) mmol/L, (0.66±0.37) mmol/L, P=0.045犦. There was no significant difference in liver and kidney function between groups (P>0.05). Conclusion: The jellyfish Saussurea cell culture pair