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目的 研究HLA -DQB1基因位点上是否存在幽门螺杆菌(H .pylori)感染及其相关胃炎的易感基因或抵抗基因,从免疫遗传角度探讨H .pylori感染后临床结局多样性的可能发生机制。方法 对1 999年9月至2 0 0 0年7月上海第二医科大学附属瑞金医院收治的1 3 3例慢性胃炎及80名健康儿童(对照组) ,进行H .pylori检测,应用PCR SSO杂交方法确定其HLA -DQB1等位基因型别。结果 80名对照组儿童中H .pylori阳性3 3名,H .pylori阴性47名;1 3 3例慢性胃炎患儿中,H .pylori阳性85例,H .pylori阴性48例。DQB1 0 3 0 3 2等位基因频率在血清学H .pylori阳性者中低于血清学H .pylori阴性的健康儿童( 1 0 . 61 %vs 2 5. 53 % ,P <0 . 0 5)。DQB1 0 60 2等位基因频率在H .pylori阳性胃炎患儿低于H .pylori阴性胃炎患儿( 4 . 71 %vs 1 2 . 50 % ,P <0 . 0 5)。结论 DQB1 0 3 0 3 2对H .pylori感染可能具有抵抗保护作用,DQB1 0 60 2缺乏可能是H .pylori相关性胃炎发生的宿主遗传因素。
Objective To investigate the presence or absence of HLA-DQB1 locus susceptibility gene or resistance gene in Helicobacter pylori infection and related gastritis, and to explore the possible mechanism of diversity of clinical outcome after H. pylori infection . Methods One hundred and thirty-three cases of chronic gastritis and 80 healthy children (control group) admitted to Ruijin Hospital Affiliated to Shanghai Second Medical University from September 1999 to July 2000 were tested for H. pylori. PCR SSO Hybridization method to determine its HLA-DQB1 allele type. Results Among 80 control children, 23 were H. pylori positive and 47 were H. pylori negative. Among 133 children with chronic gastritis, 85 were H. pylori positive and 48 were H. pylori negative. The frequency of allele DQB1 0 3 0 3 2 was lower in seroprevalence H.pylori positive than in seroprevalence H.pylori-negative healthy children (16.6% vs 25.33%, P <0.05) . The frequency of DQB1 0 60 2 allele in children with H. pylori-positive gastritis was lower than that in children with H. pylori-negative gastritis (4.71% vs 12.5%, P <0.05). Conclusion DQB1 0 3 0 3 2 may have protective effect against H.pylori infection. The lack of DQB1 0 60 2 may be the host genetic factor of H. pylori-associated gastritis.