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以自制啤酒污泥堆肥和苹果苗为试材,采用盆栽分根栽植的方式,探讨啤酒污泥堆肥不同施用量(25、50、100、200g/kg,处理代号分别为BSC1、BSC2、BSC3、BSC4)对苹果苗根系重金属富集、抗氧化酶(超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD))活性等生理特性以及根系形态的影响。结果表明:各处理隔板两边根系生长、生理特性以及重金属富集不尽相同,根系中重金属铬(Cr)、锌(Zn)、铜(Cu)和丙二醛(MDA)富集量均与啤酒污泥堆肥施用量呈正相关,且显著高于对应另一半对照;随啤酒污泥堆肥施用量增多,根系形态、抗氧化酶活性和根系活力均呈先上升后下降趋势。BSC1、BSC2、BSC3能显著促进根系生长,增加地下生物量,各处理施用污泥堆肥分别是对应另一半对照的1.40、1.69、1.24、0.75倍;苹果苗根系SOD活性大小顺序为BSC2>BSC1>BSC3>BSC4;BSC1、BSC2、BSC3苹果根系CAT活性分别比对应另一半对照高28.78%、82.25%、25.57%,BSC4比CK4降低24.22%,其中BSC2显著高于BSC1、BSC3和BSC4(P<0.05),BSC3和BSC1差异不明显;不同施用量处理对苹果根系POD活性有促进作用,分别是对应另一半对照的1.41、1.83、1.42、1.14倍,且差异显著(P<0.05)。
The brewer’s sludge compost and apple seedlings were used as test materials to study the effects of different application rates of beer sludge compost (25,50,100,200g / kg, treatment code BSC1, BSC2, BSC3, BSC4) on the physiological characteristics of root and root morphology of apple seedlings, such as the enrichment of heavy metals, the activity of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) The results showed that the growth, physiological characteristics and accumulation of heavy metals in root system on both sides of each treatment partition were not the same. The contents of Cr, Zn, Cu and MDA in roots were Beer sludge composting application rate was positively correlated, and significantly higher than the corresponding other half of the control; with the amount of beer sludge compost increased, root morphology, antioxidant enzyme activity and root activity showed a downward trend. BSC1, BSC2 and BSC3 could significantly promote root growth and increase underground biomass, respectively, which was 1.40, 1.69, 1.24 and 0.75 times that of the other half of the control, respectively. The order of SOD activity in the roots of apple seedlings was BSC2> BSC1> BSC3> BSC4; CAT activity of BSC1, BSC2 and BSC3 apples were 28.78%, 82.25% and 25.57% higher than that of the other half, respectively. BSC4 was 24.22% lower than that of CK4, and BSC2 was significantly higher than that of BSC1, BSC3 and BSC4 ), BSC3 and BSC1. There was no significant difference between the two treatments (P <0.05). The POD activity of apple roots was promoted by different application rates, which were 1.41,1.83,1.42 and 1.14 times of the control, respectively.