Experimental study on alteration of adrenergic receptors activity in neuronal membranes protein o

来源 :Journal of Medical Colleges of PLA | 被引量 : 0次 | 上传用户:caoheng19
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Objective:To definethecourseof changestakenbyα 1 andβadrenergicreceptors(AR)activityaftertraumatic braininjury(TBI)andexploretheapproachforsecondarybraininjury(SBI)management.Methods:Theneuronalmem-braneproteinof cortexwereextractedfromtheratssubjectto traumaticbraininjury,andthechangesofα 1 -andβ-ARac-tivitiesintheneuronalmembraneswereexaminedby radioligandbindingassay(RLBA).Results:α 1 -andβ-ARactivities underwentobviouschanges,reachingtheirpeakvaluesat24h afterTBI.α 1 -ARbindingdensity(B max )reducedby22.6%whiletheligandaffinityincreasedby66.7%,andforβ-AR,however,B max increasedby116.9%andtheligandaffinityre-ducedby50.7%.Theirantagonistscouldcounteractthechangesofα 1 -andβ-ARactivity.Con clu sion:Thepatternsof changesvariesbetweenα 1 -andβ-ARactivityafterTBI, suggestingtheirdifferentrolesintheneuronalmembranesafter braintrauma,andtimelyadministrationof ARantagonistsispotentiallybeneficialinTBImanagement. Objective: To definethecourseof changestakenbyα 1 andβadrenergicreceptors (AR) activityaftertraumatic braininjury (TBI) andexploretheapproachforsecondarybraininjury (SBI) management.Methods: Theneuronalmem-braneproteinof cortexwereextractedfromtheratssubjectto traumaticbraininjury, andthechangesofα 1 -andβ-ARac-tivitiesintheneuronalmembraneswereexaminedby radioligandbindingassay (RLBA) .Results: α 1 -andβ-ARactivities underwent epithelium prior to24h afterTBI.alpha.-ARbindingdensity (Bmax) reducedby22.6% while athelixandaffinity increased by66.7%, andforβ-AR, however, B max increasedby116.9% and theligandaffinityre-ducedby50.7% .Theirantagonistscouldcountethechangesofalpha-andbeta- ARactivity.Con clu sion: The Patterns of Changes Varies Between α 1 -and β-AR activity after TBI, suggesting these multiple receptors in vitro human bambranes after braintrauma, and timely administration of ARantagonists administered potential benefits in TB Management.
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