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目的:建立SD大鼠腹腔注射Ouabain诱导的弱精子症动物模型。方法:健康性成熟SD雄性大鼠40只,随机分为对照组(生理盐水,n=10),低剂量实验组(Ouabain 12.5μg/kg,n=15),高剂量实验组(Ouabain 25μg/kg,n=15),各组大鼠连续每日腹腔注射相应试剂,qd×30 d,观察各组附睾精子活力及活动率的变化,同时观察本实验剂量范围的Ouabain对大鼠生殖系统及全身可能的毒性反应。结果:与对照组相比,实验组大鼠精子数量无明显变化,但精子活力及活动率显著下降。高剂量及低剂量Ouabain组连续给药30 d后大鼠附睾精子活力分别为48.27%和44.73%,与对照组(73.33%)相比有显著性差异(P<0.01),且高、低剂量组间差异显著(P<0.01);高、低剂量组大鼠精子活动率分别为52.26%和49.95%,与对照组(78.70%)相比有显著性差异(P<0.01),高、低剂量组间亦有统计学差异(P<0.01)。本实验剂量范围内,Ouabain对大鼠生殖器官及全身重要脏器未显示明显的毒性反应。结论:Ouabain经腹腔注射给药30 d能成功诱导建立SD大鼠弱精子症模型的,且未见明显的生殖系统和全身毒性反应。
OBJECTIVE: To establish an animal model of asthenospermia induced by Ouabain in SD rats. Methods: Forty healthy male SD rats were randomly divided into control group (n = 10), low dose group (Ouabain 12.5μg / kg, n = 15), high dose group (Ouabain 25μg / kg, n = 15). The rats in each group were given daily intraperitoneal injection of the corresponding agents for qd × 30 d. The changes of sperm motility and activity of the epididymis in each group were observed. Meanwhile, the effects of Ouabain on the reproductive system and Possible systemic toxicity. Results: Compared with the control group, there was no significant change in the number of sperm in the experimental group, but the sperm motility and activity decreased significantly. Epididymal sperm motility of rats in high dose and low dose Ouabain group were 48.27% and 44.73% respectively after 30 days continuous administration, which was significantly different from that in control group (73.33%) (P <0.01) The sperm motility of high and low dose groups were 52.26% and 49.95%, respectively, which were significantly different from those of control group (78.70%) (P <0.01) There was also a significant difference between dose groups (P <0.01). Within the experimental dose range, Ouabain showed no significant toxicity to the reproductive organs and vital organs of rats. CONCLUSION: Ouabain administered intraperitoneally for 30 days can successfully induce the establishment of SD rat model of asthenospermia and showed no obvious reproductive system and systemic toxicity.