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目的建立止嗽定喘丸中盐酸麻黄碱与盐酸伪麻黄碱的含量测定方法。方法采用固相萃取技术处理样品,HPLC测定,色谱柱为Shim-pack VP-ODS C18(150 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(5∶95),检测波长210 nm,流速1 mL·min-1。结果 4.41～147.0μg·mL-1盐酸麻黄碱、2.33～116.6μg·mL-1盐酸伪麻黄碱与峰面积的线性关系良好(r=0.9999),回收率分别为100.02%、97.73%(n=9)。结论所用方法准确可靠、重复性好,可作为止嗽定喘丸的质量控制。
Objective To establish a method for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Zhuxian Dingchuan Pills. Methods The samples were determined by solid phase extraction (SPE) with HPLC. The chromatographic column was Shim-pack VP-ODS C18 (150 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile-0.1% phosphoric acid nm, flow rate 1 mL · min-1. Results The linear range was 4.41 ~ 147.0μg · mL-1 for ephedrine hydrochloride and 2.33-116.6μg · mL-1 for pseudoephedrine hydrochloride (r = 0.9999). The recoveries were 100.02% and 97.73% (n = 9) . Conclusion The method used is accurate and reliable with good repeatability and can be used as quality control of Cough Dingchuan Pills.