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目的:探讨NSCs与OECs联合移植对TBI大鼠运动功能修复的作用。方法:85只SD成年大鼠采用经典的Feeney氏法自由落体撞击法造成TBI运动功能损伤。剔除死亡及不合格的10只,余75只大鼠随机分为损伤对照组(A组)、NSCs移植组(B组)、NSCs与OECs联合移植组(C组)。各组均咬开骨窗,显露硬膜,于造模后1、7、14、25、30天进行神经功能评分并取材行HE及免疫组化染色,比较各组行为学评分及组织染色结果。结果:各组25只大鼠均进入分析,B组、C组7天、14天、25天、30天较A组有显著差异性(P<0.05),C组在25天、30天与B组比较有显著差异性(P<0.05)。结论:NSCs联合OECs移植能改善TBI大鼠运动功能,且治疗效果优于NSCs移植。
Objective: To investigate the effect of transplantation of NSCs and OECs on the motor function of TBI rats. Methods: Eighty-five SD adult rats were sacrificed by classic Feeney’s method of free-fall impact on TBI motor function. Totally 10 dead and unqualified rats were randomly divided into three groups: injured control group (A group), NSCs transplantation group (B group) and NSCs combined with OECs transplantation group (C group). Each group was bite open the window, revealing the dura, nerve function score and made HE and immunohistochemical staining at 1, 7, 14, 25, 30 days after modeling, behavioral score and histological staining results . Results: 25 rats in each group were involved in the analysis. There was significant difference (P <0.05) between group B and group C at 7 days, 14 days, 25 days and 30 days (P <0.05) B group was significantly different (P <0.05). Conclusion: The transplantation of NSCs combined with OECs can improve the motor function of TBI rats, and the treatment effect is better than NSCs transplantation.