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为获得苹果砧木逆境胁迫相关转录因子,以QZ1(平邑甜茶(Malus hupehensis Rehd.)×柱型苹果株系CO(Malus domestica Borkh.)和M26为试材,利用同源克隆法克隆得到苹果SBP基因cDNA的全长序列,命名为MdSBP20。对扩增得到的cDNA序列进行生物信息学分析,结果表明,该序列全长1 362 bp,包含完整开放阅读框1 362 bp,编码454个氨基酸,具有明显的SBP结构域,包含2个锌指结构Zn-1、Zn-2和双向核定位信号区NLS。系统进化树分析,结果表明MdSBP20与白梨(XM_009339726.1)和苹果(XM_008376435.1)亲缘关系较近。实时荧光定量分析结果表明,MdSBP20基因在低温、干旱、盐害中发挥一定功能,推测QZ1的耐寒性、耐旱性及耐盐性均强于M26。研究表明,MdSBP20基因在苹果响应非生物逆境胁迫中具有重要调控作用。
In order to obtain transcription factors related to stress stress in apple rootstocks, apple SBP was cloned by using homologous cloning method with QZ1 (Malus domestica Rehd. × columnar apple (CO) (Malus domestica Borkh.) And M26 as test materials The full-length cDNA sequence of MdSBP20 was named as MdSBP20.The bioinformatics analysis of the amplified cDNA sequence showed that the full-length cDNA was 1 362 bp in length and contained a complete open reading frame of 1 362 bp encoding 454 amino acids with The obvious SBP domain contains two zinc finger structures, Zn-1, Zn-2 and NLS. The phylogenetic tree analysis showed that MdSBP20 interacted with the white pear (XM_009339726.1) and apple (XM_008376435.1) The results of real-time fluorescence quantitative analysis showed that MdSBP20 gene was able to play a certain role in low temperature, drought and salinity damage, suggesting that the cold tolerance, drought tolerance and salt tolerance of QZ1 are stronger than M26. Apple has an important regulatory role in response to abiotic stresses.