论文部分内容阅读
银杉(Cathaya argyrophylla)是一种极其珍稀的濒危松科植物。该研究建立了一种有效的银杉人工菌根诱导技术。通过将银杉种子播种于琼脂培养基7d后,切除1/5~1/3的种皮以及胚乳,从而使银杉种子的发芽率达到60%以上;同时筛选出既适合外生菌根菌土生空团菌(Cenococcumgeophilum)平铺扩展生长的要求,又能满足银杉幼苗生长的培养基RM,使银杉的侧根能最大程度地与菌丝接触,确保了菌根的诱导率;通过紫外线照射加70%酒精或0.05%NaOCl的表面消毒的双重处理,使不能高压灭菌的容器达到表面消毒效果,同时验证了用该灭菌法既能达到完全的灭菌效果又不会对植物的生长产生不良影响,而且银杉小苗的菌根形成率达到87.5%。该方法对于银杉的菌根学研究以及其它基础研究具有实际应用价值。
Cathaya argyrophylla is an extremely rare species of endangered pine. The study established an effective silver fir artificial mycorrhizal induction technology. After the silver fir seeds were sown on the agar medium for 7 days, 1 / 5-1 / 3 of the seed coat and the endosperm were excised, so that the germination rate of the silver fir seeds reached 60% or more. At the same time, the ectomycorrhizal fungi Cenococcumgeophilum tilapia growth requirements, but also to meet the growth of silver fir seedlings medium RM, silver fir lateral root contact with maximum contact with mycelium to ensure the induction rate of mycorrhizal; by ultraviolet Irradiation plus 70% alcohol or 0.05% NaOCl surface disinfection dual treatment, so that can not be autoclaved containers to achieve the surface disinfection effect, at the same time to verify that the sterilization method can achieve both complete sterilization without the effect of plant Growth has a negative impact, and silver fir seedlings mycelium formation rate reached 87.5%. The method has practical application value for mycorrhizal fungi research and other basic research.