论文部分内容阅读
目的：探讨三氧化二砷(As2O3）的耐药机制。方法：用四甲基偶氮唑蓝(MTT）法检测A_(549)~(DDP)细胞对As_2O_3的耐药性，用逆转录聚合酶链反应(RT－PCR）技术检测As_2O_3处理后A_(549)~(DDP) 细胞多药耐药基因(mdr1）及多药耐药相关蛋白(MRP）基因表达。结果：A_(549)~(DDP) 细胞对As_2O_3具有交叉耐药性。A_(549)和A_(549)~(DDP) 细胞中mdr1基因低表达，MRP基因呈较高水平表达。结论：A_(549)~(DDP) 细胞中MRP基因过度表达可能是As_2O_3耐药的主要机制之一。
Objective: To investigate the resistance mechanism of arsenic trioxide (As2O3). METHODS: MTT assay was used to detect the drug resistance of A 549 DDP cells to As 2 O 3 , and As 2 O 3 was detected by reverse transcription polymerase chain reaction (RT-PCR). 549)DDP multidrug resistance gene (mdr1) and multidrug resistance-associated protein (MRP) gene expression. Results: A_(549)~(DDP) cells had cross-resistance to As_2O_3. The mdr1 gene was down-regulated in A_(549) and A_(549)~(DDP) cells, and the MRP gene was highly expressed. Conclusion: Overexpression of MRP gene in A_(549)~(DDP) cells may be one of the main mechanisms of As_2O_3 resistance.