从建兰花叶病毒 (CyMV)石斛兰分离物中提取病毒RNA ,用反转录———聚合酶链式反应 (RT PCR)方法获得约 5 0 0bp的运动蛋白基因片断 ,插入 pGEM T载体克隆并测序。序列分析表明 ,该基因片断由 474个核苷酸组成 ,和CyMV美国夏威夷分离物、新加坡分离物相应基因核甘酸序列分别具有 97.8%同源性 ;根据核酸序列推导该片断含有 3个部分重叠的开放阅读框架 (ORF) ,分别编码 14kD、12kD和 10kD的多肽 The viral RNA was extracted from the Cymbidium candidum (CyMV) Dendrobium spp., And about 500 bp of the motor protein gene fragment was obtained by reverse transcription-polymerase chain reaction (RT PCR). The pGEM T vector clone And sequenced. Sequence analysis showed that this gene fragment consisted of 474 nucleotides, which had 97.8% homology with the corresponding nucleotide sequence of CyMV Hawaii isolates and Singapore isolates respectively. The deduced amino acid sequence of the fragment contained 3 partially overlapping Open reading frames (ORFs) encoding polypeptides of 14 kD, 12 kD and 10 kD, respectively