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本文用薄层色谱一分光光度法测定黛芩化痰丸中靛玉红和黄芩甙含量,靛玉红的分析是在硅胶G薄层板上,用苯-醋酸乙酯-甲酸-水(60:20:1.00:10)为展开剂,分离的斑点以氯仿提取,用550 nm波长测定吸收度,计算含量。回收率为98.01％,变异系数为1.3％。黄芩甙的测定是在硅胶GF_254薄层板上,用正丁醇-乙酸-水(4:1.5:4.2)为展开剂,分离的斑点用紫外分析仪定位后,以甲醇-水(9:11)提取,用276.0nm波长测定吸收度,计算含量。回收率为100.9％,变异系数为0.94％。
In this paper, the contents of indirubin and astragalus in Bianhuayu Pills were determined by TLC-spectrophotometry. The analysis of indirubin was on silica gel G plate, using benzene-ethyl acetate-formic acid-water (60). : 20:1.00:10) As a developing agent, the separated spots were extracted with chloroform, the absorbance was measured at a wavelength of 550 nm, and the content was calculated. The recovery rate was 98.01% and the coefficient of variation was 1.3%. Astragalus membranaceus was determined on a silica gel GF_254 thin-layer plate using n-butanol-acetic acid-water (4:1.5:4.2) as a developing solvent. The separated spots were positioned with an ultraviolet analyzer and methanol-water (9:11) was used. ) Extraction, determination of absorbance at 276.0 nm wavelength, calculation of content. The recovery rate was 100.9% and the coefficient of variation was 0.94%.