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目的：探讨血小板源性生长因子（ＰＤＧＦ）在低氧肺动脉高压血管构形重建发生中的作用。方法：应用免疫组化技术结合计算机图像分析，检测了低氧大鼠腺泡内肺动脉（ＩＡＰＡ）ＰＤＧＦ－Ｂ链蛋白表达水平。结果：常氧时，ＩＡＰＡ仅有ＰＤＧＦ－Ｂ链蛋白弱表达；低氧１天时，ＩＡＰＡ便有较强的ＰＤＧＦ－Ｂ链蛋白表达，定位于ＩＡＰＡ的内皮和中膜，低氧３天至１４天仅分布于中膜；低氧１、３、５、７、１４天各时间点ＰＤＧＦ－Ｂ链蛋白表达分别为常氧组的１．５３、１．５９、１．５６、１．６２和１．４２倍，差异有显著性（Ｐ＜０．０１）。结论：ＰＤＧＦ－Ｂ链蛋白可能参与了低氧肺动脉高压血管构形重建的发病过程
Objective: To investigate the role of platelet derived growth factor (PDGF) in the angiogenesis of hypoxic pulmonary hypertension. Methods: Immunohistochemistry and computer image analysis were used to detect the expression of PDGF-B protein in pulmonary artery (IAPA) of hypoxic rats. Results: Under normoxia, only PDGF-B chain protein was weakly expressed in IAPA. When hypoxia for 1 day, IAPA had stronger expression of PDGF-B chain protein, located in the endothelium and intima of IAPA, hypoxia for 3 days to 14 Day only distributed in the media; 1, 3, 5, 7, 14 days hypoxia at each time point PDGF-B chain protein expression were nocturnal 1.53,1.59,1.56,1.62 and 1.42 times, the difference was significant (P <0.01). Conclusion: PDGF-B chain protein may be involved in the pathogenesis of hypoxia-induced pulmonary hypertension